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KMID : 0545120070170061049
Journal of Microbiology and Biotechnology
2007 Volume.17 No. 6 p.1049 ~ p.1053
Optimization of Tannase Production by Aureobasidium pullulans DBS66
DEBDULAL BANERJEE

Pati Bikas R.
Abstract
Tannase production by Aureobasidium pullulans DBS66 was optimized. The organism produced maximum tannase in the presence of 1% tannic acid after 36 h. Maximum gallic acid accumulation was observed within 36 h and tannic acid in the fermented broth was completely degraded after 42 h of growth. Glucose had a stimulatory effect on tannase synthesis at 0.1 % (w/v) concentration. The organism showed maximum tannase production with (NH4)2HPO4 as nitrogen source. Shaking speed of 120 rpm and 50-ml broth volume have been found to be suitable for maximum tannase production.
KEYWORD
Aureobasidium pullulans, gallotannin, optimization, tannase, yeast
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