KMID : 0545120070170061049
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Journal of Microbiology and Biotechnology 2007 Volume.17 No. 6 p.1049 ~ p.1053
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Optimization of Tannase Production by Aureobasidium pullulans DBS66
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DEBDULAL BANERJEE
Pati Bikas R.
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Abstract
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Tannase production by Aureobasidium pullulans DBS66 was optimized. The organism produced maximum tannase in the presence of 1% tannic acid after 36 h. Maximum gallic acid accumulation was observed within 36 h and tannic acid in the fermented broth was completely degraded after 42 h of growth. Glucose had a stimulatory effect on tannase synthesis at 0.1 % (w/v) concentration. The organism showed maximum tannase production with (NH4)2HPO4 as nitrogen source. Shaking speed of 120 rpm and 50-ml broth volume have been found to be suitable for maximum tannase production.
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KEYWORD
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Aureobasidium pullulans, gallotannin, optimization, tannase, yeast
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